Native-page: everything in-tact, separated by size and charge or shape.
ie. If we had a heterotrimer, we only would see one band.
SDS-page non-reducing(w/o B-ME): S-S are intact but protein is denatured
ie. If we had a heterotrimer, we would only see one band.
SDS-page reducing (w/ B-ME): S-S become reduced protein is still dentured.
ie. If we had a heterotrimer, we would see 3 separate bands....
SDS-page reducing vs. non-reducing vs. native
ie. If we had a heterotrimer, we only would see one band.
SDS-page non-reducing(w/o B-ME): S-S are intact but protein is denatured
ie. If we had a heterotrimer, we would only see one band.
SDS-page reducing (w/ B-ME): S-S become reduced protein is still dentured.
ie. If we had a heterotrimer, we would see 3 separate bands....
SDS-page reducing vs. non-reducing vs. native