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EK FL 1: pKa and Protein Stability

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"Which of the following residues if replaced with a neutral leucine residue would cause the protein to become the most stable?

a) D12
b) D48
c) E6
d) E47

Explanation: The passage states that the change in pKa closely reflects the change in G between the unfolded and unfolded protein states. Therefore, the residue with the largest change in pKa in the positive direction will destabilize the protein the most."

Graph is attached. I got this question correct just based on "one of...

EK FL 1: pKa and Protein Stability

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