My question is about how to choose the solvent (from among mercaptoethanol, urea, or GuHCl) to create a favorable environment for activity of a hydrophobic enzyme. I think I'm not understanding this question because I don't really get how these solvents would (or would not) interact with something hydrophobic.
1. I'm trying to understand this in terms of like-dissolves-like - is this how I should be thinking about this?
2. Since beta-galactosidase is mostly hydrophobic, do we want to use...
EK FL 3 #37
1. I'm trying to understand this in terms of like-dissolves-like - is this how I should be thinking about this?
2. Since beta-galactosidase is mostly hydrophobic, do we want to use...
EK FL 3 #37