Both are measures of affinity.
I would like clarifications as to how they are different.
My understanding is the following:
Km: The concentration of substrate at which half of the active sites are full.
Smaller Km indicates a higher affinity because it means you need less substrate to reach half full due to them being attracted.
Ka: [ Substrate enzyme complex] / [Enzyme concentration]
So a higher Ka would indicate that for the same enzyme concentration, the enzyme would be more...
Ka vs Km
I would like clarifications as to how they are different.
My understanding is the following:
Km: The concentration of substrate at which half of the active sites are full.
Smaller Km indicates a higher affinity because it means you need less substrate to reach half full due to them being attracted.
Ka: [ Substrate enzyme complex] / [Enzyme concentration]
So a higher Ka would indicate that for the same enzyme concentration, the enzyme would be more...
Ka vs Km