Tissue staining with specific antibodies, known as immunohistochemical analysis, did NOT show increased levels of pStat3 (phosphorylated Stat3 transcription factor). What explanation is LEAST likely to account for this finding?
A. pStat3 is denatured during the tissue preparation process
B. Low grade primary tumors were stained instead of malignant tumors
C. Anti-pStat3 antibody also recognizes pStat5 (correct)
D. A secondary antibody-enzyme conjugate was not used in the experiment...
EK - antibody specificity
A. pStat3 is denatured during the tissue preparation process
B. Low grade primary tumors were stained instead of malignant tumors
C. Anti-pStat3 antibody also recognizes pStat5 (correct)
D. A secondary antibody-enzyme conjugate was not used in the experiment...
EK - antibody specificity