Hello everyone, I have an issue with this question I hope someone can clarify for me
View attachment 218422
So, I understand that Histidine shouldn't have a positive charge here on the bases that it's side chain's pKa is ~6 and the pH is stated to be 7.... but what about the pI? Since both the amine and side chain are exposed on the histidine shouldn't the pI be ~7.59 which would mean that it's protonated?
Thanks
View attachment 218422
So, I understand that Histidine shouldn't have a positive charge here on the bases that it's side chain's pKa is ~6 and the pH is stated to be 7.... but what about the pI? Since both the amine and side chain are exposed on the histidine shouldn't the pI be ~7.59 which would mean that it's protonated?
Thanks