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Ion Exchange Chromatography Question

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A mix of Cytochrome C, Myoglobin, and Alkaline phosphatase are placed into a gel chromatography column filled with DEAE, a positively charged stationary phase. 2 different Tris buffers at pH of 8 are used to elute different fractions. The first buffer has a NaCl concentration of 0.15 molar, while buffer 2 has a NaCl concentration of 0.5 molar. Of the three collected fractions, which protein for the original mix would end up in which fraction?

Basically, which of the 3 proteins elutes out in...

Ion Exchange Chromatography Question

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