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Is there typically a minimum concentration of protein needed for detection? For the RAD51 control cases, for example, would it be valid to say that no RAD51 is present? Or could it just be in very low concentrations that the blot didn't pick it up.
Is there typically a minimum concentration of protein needed for detection? For the RAD51 control cases, for example, would it be valid to say that no RAD51 is present? Or could it just be in very low concentrations that the blot didn't pick it up.